Save as supportAutoCAD R14, AutoCAD 2000, AutoCAD 2004, AutoCAD 2007, AutoCAD 2010, AutoCAD 2013, AutoCAD 2018.EMR has the same formats as Commercial AutoCAD, with the exception of AutoCAD 2000/LT2000 Drawing*AutoCAD Architecture and AutoCAD MEP do not support Save As*AutoCAD Mechanical: AutoCAD Mechanical 6, AutoCAD Mechanical 2004, AutoCAD Mechanical 2004DX, AutoCAD Mechanical 2005,AutoCAD Mechanical 2006, AutoCAD Mechanical 2007, AutoCAD Mechanical 2008, AutoCAD Mechanical 2009, AutoCAD Mechanical 2010,AutoCAD Mechanical 2011, AutoCAD Mechanical 2012, AutoCAD Mechanical 2013, AutoCAD Mechanical 2014, AutoCAD Mechanical 2015,AutoCAD Mechanical 2016, AutoCAD Mechanical 2018, AutoCAD Mechanical 2021, AutoCAD Mechanical 2022, AutoCAD Mechanical 2023
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The single-etched layer of the microfluidic card also reduces costs and complexity of microfabrication. All channels were etched with greater than or equal to 200 μm dimensions to prevent clogging with dust and permit fabrication outside of a clean room. Total material costs of the acrylic layer and silicon adhesive tape is approximately $0.83/card. Costs required for reaction constitutes is approximately $0.043/ μL, which is $6.93 to fill the 64 reaction well card. For producing the airlock card on a more cost effective basis (without the CO2 laser), the 64 well version of the airlock card was also tested for fabrication via injection molding (Benchtop Injection Molding Machine, Medium Machinery LLC) with polypropylene pellets (PPBLK, LNS Technologies) casted directly with loading ports that can be sealed with screw-caps (mold fabricated by Herman Engineering, Fig. 2c). Mass production replicate techniques such as embossing and injection molding have been described previously for reducing microfluidic fabrication costs (Dang et al. 2005; Martynova et al. 1997). Tests with both acrylic and polypropylene cards fabricated with lasers and injection molding further demonstrate that the airlock card may be adaptable to numerous plastic substrates. For even lower detection costs, endpoint images can be captured (for presence/absence) using a CCD camera, which only requires a green LED and orange filter lenses for excitation and emissions, respectively. 2ff7e9595c
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